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An entire medium modify was done every day, as well as the cells ended up break up once again at the time a confluency of 80% was achieved. All undifferentiated iPSC and human ESC colonies had been managed in Critical 8 medium (E8 medium; Lifestyle Systems) and incubated at 37°C in 5% CO2 humidified incubators. iPSCs and ESCs ended up managed in T